|Family|| Anna Twombly (Great-great-granddaughter)|
Dog, Hedgehog, Skunk, Monkey, Panda, Mongoose and Gecko (Pets)
|Debut||The Treasure of Henrietta Twombly|
|Voiced by||Kathleen Barr|
Henrietta Twombly is the great-great-grandmother of Anna Twombly. She also created the first Littlest Pet Shop in the old west and the shop was passed down throughout generations. Also shown in The Treasure of Henrietta Twombly, she kept a treasure chest full of fancy pet food for the pets.